Sequencing

• Rolling Circle Amplication
• Quantiation of DNA
• Sequencing of PCR product
• Sequencing Reaction Setup
• Ethanol/EDTA/Sodium Acetate Precipitation for Big Dye v. 3.1 Chemistry
• ABI 3730xl DNA Analyzer Operation & Maintenance
• Hydra Usage
• Large Construct Isolation Protocol
• Shotgun Subcloning

Fragment Analysis

• Fragment analysis protocol

Q-Bot

• Q-BOT General Information
• Picking
• Replicating
• Gridding
• Filter Processing Solutions
• Filter Processing
• Cleaning Heads
• Freezing Medium
• Q-Tray Media
• Using the Q-Fill